APPLICATION OF EXTRACT OF PLANT TO CONTROL THE DRUG RESISTANT BACTERIA

Abstract

The three strains of bacteria that were used in the experiment were Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The purpose of the research was to evaluate the antibacterial activity of various medicinal plant extracts and their synergistic antibiotic and non-antibiotic medications. For the preparation of the alcoholic extract, a Soxhlet apparatus was used, and for the preparation of the aqueous extract, a water reflux apparatus was utilized. Both the disc diffusion technique and the well diffusion method were used in order to assess the antibacterial properties of the extracts; the inhibitory zones were measured in millimeters. The microdilution technique was used to determine the minimum inhibitory concentration (MIC) of plant extracts against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The disc diffusion technique was used in order to evaluate the synergistic impact that plants have when combined with the extraction of antibiotics and/or non-antibiotic medications. The findings of this investigation indicated that ethanolic extracts, when employed against E. coli, S. aureus, and P. aeruginosa, exhibited an antibacterial impact and a synergistic effect with the majority of antibiotics. These extracts performed much better than methanolic and aqueous extracts.

Keywords

Plant, Control, Drug, Paracetamol, Hcl