Formulation and evaluation of mupirocin loaded invasomes for effective treatment of skin infections

Abstract

Invasomes are novel vesicular systems that exhibit improved transdermal penetration compared to conventional liposomes. These vesicles contain phospholipids, ethanol, and terpene in their structures; these components confer suitable transdermal penetration properties to the soft vesicles. The main advantages of these nanovesicles lie in their ability to increase the permeability of the drug into the skin and decrease absorption into the systemic circulation, thus, limiting the activity of various drugs within the skin layer. The purpose of this work was to prepare and characterize invasomes carrier for Mupirocin, and to evaluate the optimized formulation obtained for pharmacodynamic study. Mupirocin -loaded invasomes were prepared by mechanical dispersion technique using Soya phosphatidylcholine, terpene and ethanol. Prepared formulations were characterized in terms of Entrapment efficiency, Particle Size, Drug Content, Vesicular Size and Shape and In vitro drug release.  It was observed that prepared Mupirocin-loaded invasomes delivers reasonable entrapment efficiency, and more effectiveness for transdermal delivery. Among all 6 formulation the optimized formulation F3 exhibits a mean particle size of 359.58 nm, entrapment efficiency of 85.29%, and drug content of 98.28%. These values collectively suggest that F3 is a promising formulation with desirable characteristics for drug delivery. The in-vitro drug release data of Mupirocin from F3 follows Higuchi kinetics. This implies controlled release through diffusion as the dominant mechanism.

Keywords

Invasomes, Mupirocin, Mechanical Dispersion, Soya phosphatidylcholine